- Phillip Hannon as Mario
- Jean Lennox as Princess Peach
- Bob Matthews as Luigi
- Inspector Grovening as Yoshi
- Lady Syrett as Birdo
- Barmaid as Princess Daisy
- Mr. Murch as Toadsworth
- Pillings as Wario
- Janet Murch as Toadette
- Sergeant Luce as Donkey Kong
- Miss Alice MacDonald as Princess Rosalina
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