- Jackie Robinson as Wakko
- Branch Rickey as Skippy Squirrel
- Wendell Smith as Dr. Otto
- Leo Durocher as Thaddeus Plotz
- Red Barber as Mr. Director
- Clyde Sukeforth as Mr. Skullhead
- Pee Wee Reese as Squit
- Ben Chapman as Buttons
- Rachel Isum Robinson as Dot
- Happy Chandler as Yakko
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