- Agent Cody Banks as Kermit the Frog
- Natalie Katerina Connors as Annie Sue Pig
- Ronica Miles as Miss Piggy
- CIA Director as Jim Henson
- Dr. Brinkman as Uncle Deadly
- Francois Molay as Gonzo the Great
- Dr. Albert Connors as Floyd Pepper
- Mrs. Banks as Janice
- Mr. Banks as Animal
- Alex Banks as Dr. Bunsen Honeydew
- Earl as Beaker
- Rosychuk as Robin the Frog
- Fenster as Sam the Eagle
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