- Agent Cody Banks as Ash Ketchum
- Natalie Katerina Connors as Dawn
- Ronica Miles as May
- CIA Director as Professor Birch
- Dr. Brinkman as Mewtwo
- Francois Molay as Pikachu
- Dr. Albert Connors as Todd
- Mrs. Banks as Zoey
- Mr. Banks as Brock
- Alex Banks as Max
- Earl as Tracey
- Rosychuk as Richie
- Fenster as Buizel
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