- John Grady Cole as Mario
- Lacey Rawlins as Luigi
- Jimmy Blevins as Wario
- Alejandra Villarreal as Princess Daisy
- Don Hector de la Rocha as Waluigi
- Cole as Donkey Kong
- Captain as Diddy Kong
- Doña Alfonsa as Princess Peach
- The Judge as Bowser
- J.C. Franklin as Bowser Jr.
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