- Nicolò (Nickie) Ferrante as Mario
- Terry McKay as Princess Rosalina
- Kenneth Bradley as Luigi
- Lois Clark as Princess Daisy
- Grandmother Janou as Birdo
- Himself - Announcer as Toad
- Ned Hathaway as Donkey Kong
- Courbet as Bowser
- Terry McKay as Princess Peach (singing voice)
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