- Fievel as Mario
- Mike (from Monsters, Inc.) as Luigi
- Disgust (from Inside Out) as Peach
- Tiger as Yoshi
- Miss Kitty as Birdo
- Tony Toponi as Bowser
- Cappy (from Robots) as Daisy
- Princess Poppy (from Trolls) as Rosalina
- Shrek (from Shrek) as Wario
- Anger (from Inside Out) as Waluigi
- Z (from Antz) as Toad
- Moana (from Moana) as Toadette
- Po (from Kung Fu Panda) as Donkey Kong
- Blu (from Rio) as Diddy Kong
- Donkey (from Shrek) as Bowser Jr.
- Flik (from A Bug's Life) as Larry Koopa
- Dodger (from Oliver and Company) as Morton Koopa Jr.
- Elsa (from Frozen) as Wendie O. Koopa
- Sid (from Ice Age) as Iggy Koopa
- Nemo (from Finding Nemo) as Roy Koopa
- Lightning McQueen (from Cars) as Lemmy Koopa
- Basil (from The Great Mouse Detective) as Ludwig Von Koopa
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