- Einar Gikyson as Mario
- Jean Gilkyson as Princess Rosalina
- Mitch Bradley as Luigi
- Sheriff Crane Curtis as Toad
- Kitty as Princess Peach
- Gary Winston as Yoshi
- Nina as Princess Daisy
- Griff Gilkyson as Wario
- Griffin Gilkyson as Donkey Kong (in flashbacks)
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