• Yakko as Batman
  • Wakko as Robin
  • Dot as Batgirl
  • Dr. Otto ScratchNSniff as Alfred
  • Katie Ka-Boom as Aunt Harriet
  • King Acorn (from Sonic The Hedgehog) as Commissioner Gordon
  • Ralph as Chief O'Hara
  • Walter Wolf as Joker
  • Mr. Plotz as Penguin
  • Slappy Squirrel as Catwoman
  • Miss Flamiel as Poison Ivy
  • Newt as Riddler
  • Dr. Robotnik (from Adventures of Sonic The Hedgehog) as Two-Face
  • The Brain as Bane
  • Sid the Squid as Mr Freeze
  • Montana Max (from Tiny Toon Adventures) as Mad Hatter
  • Mr. Director as Mayor John Liseed

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