• Dot Warner as Dora
  • Wakko Warner as Boots
  • Runt as Benny
  • Rita as Isa
  • Skippy Squirrel as Tico
  • Pinky as Swiper
  • Bobby, Pesto, and Squit as Fiesta Trio
  • Thaddeus Plotz as Boots' Dad
  • Solley the Godpigeon as Señor Toucan
  • Brain as Grumpy Old Troll
  • Minerva Mink as Big Red Chicken
  • Baby Bluebird as Baby Bluebird
  • Buttons as Perrito
  • Phar Fignewton as Mama Jaguar
  • Chicken Boo as Baby Jaguar
  • Yakko Warner as Diego
  • Dr. Otto Scratchansniff as Dora's Dad
  • Hello Nurse as Dora's Mom
  • Miss Flamiel as Dora's Grandma
  • Dr. Jane Embryo as La Maestra
  • Mr. Director as King Juan El Bobo

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