• Yakko as Mario
  • Dot as Princess Peach
  • Walter Wolf as Bowser
  • Wakko as Yoshi
  • Pinky as Lakitu Bros.
  • Skippy Squirrel as Toad
  • King Salazar as King Bob-Omb
  • Beanie the Brain-Dead Bison as King Whomp
  • Robot as Big Bully
  • Duke the Squirrel as Big Boo
  • Death as Mr. Big I
  • The Brain as Chill Bully
  • Slappy Squirrel as Wiggler
  • Robots and Badniks (From Sonic) as Bowser's Minions

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