- Dr. Otto ScratchNSniff as Luigi
- Yakko Warner as Mario
- Flavio Hippo as Donkey Kong
- Ralph the Guard as Link
- Slappy Squirrel as Samus
- Thaddeus Plotz as Captain Falcon
- Skippy Squirrel as Ness
- Wakko Warner as Yoshi
- Squit as Kirby
- Bobby as Fox
- Pesto as Pikachu
- Dot Warner as Jigglypuff
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