- Yakko Warner as Rooney Doodle
- Dot Warner as Deedee Doodle
- Wakko Warner as Moe Doodle
- Dr. Otto Scratchansniff as Bus Driver Bob
- Hello Nurse as Jazzmin
- Minerva Mink as Mazz
- Slappy Squirrel as Jumping Judy
- Runt as Audio Murphy
- Rita as Mudge
- Chicken Boo as Mr. Moosehead
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