- Yakko as Mario
- Wakko as Luigi
- Skippy Squirrel as Toad
- Dot as Princess Toadstool
- Walter Wolf as King Koopa
- The Brain as Mouser
- Pesto, Bobby & Squit as Tryclyde
- Snowball as Koopa Troopa
- Duke the Squirrel as Clawgrip
- Dr. Qwark (From Sonic) as Fryguy
- Slappy Squirrel as Birdo
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