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- SpongeBob SquarePants as Hercules
- Toaster (The Brave Little Toaster) as Young Hercules
- XJ-4 (My Life as a Teenage Robot) as Megera
- Aladdin as Zeus
- Jasmine (Aladdin) as Hera
- Plankton (SpongeBob SquarePants) as Hades
- Yogi Bear, Boo Boo Bear, Snagglepuss, Huckleberry Hound and Quick Draw McGraw as Pain and Panic
- Edmond (Rock a Doodle) as Phil
- Twilight Sparkle, Fluttershy, Rarity, Applejack, Pinkie Pie and Rainbow Dash (My Little Pony: Friendship is Magic) as the Muses
- Queen Chrysalis (My Little Pony: Friendship is Magic), Little Miss Scary (The Mr. Men Show) and Madame Mousey (An American Tail: The Mystery of the Night Monster) as the Fates
- Doc Hudson (Cars), Tuck (My Life as a Teenage Robot), Mother Gothel (Tangled), Gypsy (A Bug's Life), Itchy (All Dogs Go to Heaven) and others as the Townfolks