• Jozel Gabcik as Mario
  • Jan Kubis as Luigi
  • Marie Kovarnikova as Princess Peach
  • Lenka Fafkova as Princess Daisy
  • Adolf Opalka as Donkey Kong
  • Uncle Hajsky as Toad
  • Mrs. Moravec as Birdo
  • Ladislav Vanek as Yoshi
  • Ata Moravec as Wario
  • Josef Bublik as Diddy Kong
  • Karel Curda as Oogtar
  • Jan Hruby as Mighty Plumber
  • Josef Valcik as Mallow
  • Jaroslav Svarc as Geno
  • Dr. Eduward as Toadsworth
  • Reinhard Heydrich as Zeekeeper

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