• Jozel Gabcik as Basil
  • Jan Kubis as Dawson
  • Marie Kovarnikova as The Queen Mouse
  • Lenka Fafkova as The Singing Female Mouse
  • Adolf Opalka as Sherlock Holmes
  • Uncle Hajsky as Toby
  • Mrs. Moravec as OIivia Flaversham
  • Ladislav Vanek as Hiram Flaversham
  • Ata Moravec as Dr. Watson
  • Karel Curda as Fidget

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