- Jozel Gabcik as Basil
- Jan Kubis as Dawson
- Marie Kovarnikova as The Queen Mouse
- Lenka Fafkova as The Singing Female Mouse
- Adolf Opalka as Sherlock Holmes
- Uncle Hajsky as Toby
- Mrs. Moravec as OIivia Flaversham
- Ladislav Vanek as Hiram Flaversham
- Ata Moravec as Dr. Watson
- Karel Curda as Fidget
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