- Sterling Malory Archer as Mario
- Lana Kane as Princess Rosalina
- Malory Archer as Princess Peach
- Cryril Figgis as Yoshi
- Cheryl Tunt as Princess Daisy
- Pamela "Pam" Poovey as Birdo
- Mr. Doctor Algernop as Luigi
- Ray Gillette as Toad
- Woodhouse as Donkey Kong
- Barry Dylan as Bowser
- Len Trexler as Wario
- Nikolai Jakov as Bowser Jr.
- Mannfred as Waluigi
- Uta as Toadiko
- Trinette Magoon as Toadette
- and more
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