- Milo Thatch as Mario
- Princess Kida as Princess Peach
- Dr. Sweet as Donkey Kong
- Mole as Yoshi
- Vinny as Oogtar
- Preston Whitmore as Luigi
- Cookie as Toad
- Whelmina Packard as Princess Daisy
- Helga Sinclair as Wendy O. Koopa
- Lyle T. Rourke as Bowser
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