- Tony Stark/Iron Man as Gene
- Steve Rogers/Captain America as Fix-It Felix Jr.
- Dr. Bruce Banner/Hulk as Wreck-It Ralph
- Thor as Dr. Eggman
- Natasha Romanoff/Black Widow as Sergeant
- Sam Wilson/Falcon as Norwood
- Ultron as Sour Bill
- Wanda Maximoff/Scarlet Witch as Mary
- James Rhodes/War Machine as Root Beer Tapper
- Vision as Clyde
- Heimdall as Bowser
- Laura Barton as Deanna
- Erik Selvig as General Hologram
- World War Veteran ll as Bowser
- Dr. Helen Cho as Vanellope
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