- Cody Zamora as Daisy Duck
- Anita Crown as Minnie Mouse
- Eileen Spenser as Clarabelle Cow
- Lily Laronette as Clara Cluck
- Kid Jarrett as Mickey Mouse
- William Tucker as Professor Ludwig Von Drake
- Frank Jarrett as Donald Duck
- Josh McCoy as Goofy
- Pinkerton Detective Graves as Chip
- Pinkerton Detective O'Brady as Dale
- Preacher Sloan as Casey Junior
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