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- Barney as Basil
- Cody as Dr. Dawson
- Baby Bop as Olivia
- BJ as Flaversham
- Riff as Toby
- Luci as Mrs. Judson
- Tosha as The Mouse Queen
- Carlos as Sherlock Holmes
- Browser(from Super Mario Bros.) as Professor Ratigan
- Iron' Arry and Iron' Bert(from Thomas the Tank Engine) as Fidget
- Whickersham Monkeys(from Horton Hears A Who!) as Ratigan's Henchman