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Ferb Fletcher (Phineas and Ferb) as Batman/Bruce Wayne
Phineas Flynn (Phineas and Ferb) as Robin/ Dick Grayson
Dr. Doofenshmirtz (Phineas and Ferb) as The Joker
The Coachman (Pinocchio) as The Penguin
Candace Flynn (Phineas and Ferb) as Harley Quinn
Vanessa Doofenshmirtz (Phineas and Ferb) as Catwoman
Captain Hook (Peter Pan) as Two-Face
Plankton ( SpongeBob Squarepants) as Mr. Freeze
The Summerween Trickster (Gravity Falls) as Scarecrow
Fire Lord Ozai (Avatar: The Last Airbender) as Ra's Al Ghul
Frollo (The Hunchback of Notre Dame) as Hugo Strange
Lawrence Fletcher (Phineas and Ferb) as Alfred Pennyworth
Major Monogram (Phineas and Ferb) as Commissioner Gordon