- Commander Alex Hopper as Bugs Bunny
- Commander Stone Hopper as Daffy Duck
- Cora Raikes as Lola Bunny
- Samantha "Sam" Shane as Petunia Pig
- Captain Nagata as Taz
- Cal Zapata as Tweety Bird
- Admiral Terrance Shane as Yosemite Sam
- The U.S. Secretary of Defense as Marvin the Martian
- Walter "Beast" Lynch as Road Runner
- Lieutenant Colonel Mick Canales as Barnyard Dawg
- Dr. Nogrady as Speedy Gonzales
- Jimmy "Ordy" Ord as Pepe Le Pew
- Chief Engineer Hiroki as Sylvester
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