• Commander Alex Hopper as Mario
  • Commander Stone Hopper as Luigi
  • Cora Raikes as Princess Peach
  • Samantha "Sam" Shane as Princess Daisy
  • Captain Nagata as Toad
  • Cal Zapata as Yoshi
  • Admiral Terrance Shane as Bowser
  • The U.S. Secretary of Defense as Larry Koopa
  • Walter "Beast" Lynch as Wario
  • Lieutenant Colonel Mick Canales as Donkey Kong
  • Dr. Nogrady as Oogtar
  • Jimmy "Ordy" Ord as Diddy Kong
  • Chief Engineer Hiroki as Toadsworth

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