- Charlie Brown (from Peanuts) as Jake
- Lucy Van Pelt (from Peanuts) as Izzy
- Diego (from Go, Diego, Go!) as Cubby
- Tweety (from Looney Tunes) as Skully
- Sheldon J. Plankton (from Spongebob Squarepants) as Captain Hook
- Taz (from Looney Tunes) as Mr. Smee
- Sly the Fox as Sharky
- Crunch the Shark as Bones
- Pinky the Flamingo as Red Jessica
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