- Diego (from Go, Diego, Go!) as Mario
- Charlie Brown (from Peanuts) as Luigi
- Thumper (from Bambi) as Yoshi
- Dora (from Dora the Explorer) as Princess Peach
- Lucy Van Pelt (from Peanuts) as Princess Daisy
- Pinky the Flamingo as Birdo
- Daffy Duck (from Looney Tunes) as Wario
- Mickey Mouse (from Mickey Mouse) as Toad
- Minnie Mouse (from Mickey Mouse) as Toadette
- Sheldon J. Plankton (from Spongebob Squarepants) as Bowser
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