• Joe as Miguel
  • Steve as Tulio
  • Ms. Pepper as Chel
  • Green Puppy, Mr. Salt, Tickety Tock, Pail, Toni Braxton, Shovel, Mailbox, Barney (from Barney), Bear (from Bear in the Big Blue House), Snail, Timon and Pumbaa (from The Lion King) as El Dorado Villagers
  • Tyrese Gibson as Altivo
  • Gaston (from Beauty and the Beast) as Tzekel-Khan
  • Vector (from Despicable Me) as Cortes
  • Kong (from King Kong (1976)) as Giant Stone Jaguar
  • Blue as Bibo the Armadillo
  • Periwinkle and Cinnamon as Two Kids
  • Tia and Tamera Mowry as Two Girls in Barcelona
  • Jafar (from Aladdin) as Spanish Guard

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