• State's Atty. Henry L. Harvey as Mario
  • Madge Harvey as Princess Daisy
  • Chief Harold F. Robinson asLuigi
  • Irene Nelson as Princess Beach
  • John Waldron as Wario
  • Dave Woods as Yoshi
  • T.M. Wade as Toad
  • Mac McCreery as Donkey Kong
  • Paul Harris as Waluigi
  • Det. Lt. White as Diddy Kong
  • Stone as Oogtar
  • Whitney as Boo
  • A Suspect in the Police Line-Up as Kamek (uncredited)

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