- Alice Marano as Princess Peach
- Darlene Davis as Birdo
- Henry Greene as Mario
- Roy Knox as Yoshi
- Yon Greene as Princess Daisy
- Nick Parks as Luigi
- Doug Davis as Diddy Kong
- Beth Ann Gardener as Toadette
- Bill Marano as Oogtar
- Chief Detective Jagkrit as Donkey Kong
- Jason as Wario
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