- Gil as Diego
- Deema as Alicia
- Mrs. Pinkytoe as Click The Camera
- Oona as Daisy/Backpack
- Farmer Joe as Diego's Papi
- Ms. Jenny as Diego's Mami
- Molly as Dora (from Dora The Explorer)
- Bubble Puppy as Baby Jaguar
- Goby as Rescue Pack
- The Rockband Lobsters as The Bobo Brothers
- The rest of Bubble Guppies characters as other animals and persons
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