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- Caillou as Basil
- Leo as Dr. Dawson
- Rosie as Olivia Flaversham
- Boris as Hiram Flaversham
- Doris as Mrs. Jusdon
- Tai Lung from(Kung Fu Panda) as Professor Ratigan
- Butch from(Tom and Jerry) as Fidget
- The Wichersham Brothers from(Dr. Seuss Horton Hears A Who! 2008) as Ratigan's Henchmen
- The Wicked Weasel from(The Berenstain Bears) as Felicia the Cat
- Pumbaa from(Disney's The Lion King) as Toby the Dog
- Kevin as The Singing Female Mouse
- Sarah as Queen Mousetoria
- Mr. Washinton as The Bar Men