• Captain America/Steve Rogers as Mario
  • Iron Man/Tony Stark as Oogtar
  • Crossbones/Brock Rumlow as Waluigi
  • Black Widow/Nastasha Romanoff as Princess Peach
  • Hawkeye/Clint Barton as Diddy Kong
  • The Falcon/Sam Wilson as Toad
  • Winter Soldier/Bucky Barnes as Wario
  • Black Panther/T'Challa as Troff/Scoff
  • Baron Helmut Zemo as Bowser
  • Agent 13/Sharon Carter as Princess Daisy
  • Thunderbolt Ross as Donkey Kong
  • Ant-Man/Scott Lang as Suy Guy
  • James Rhodes/War Machine as Luigi
  • Vision as Chunky Kong
  • Scarlet Witch/Wanda Maximoff as Pauline
  • Spider-Man/Peter Parker as Yoshi
  • Christine Everhart as Birdo
  • Ellen Brandt (from Iron Man 3) as Wendy O. Koopa
  • Peggy Carter as Rosalina

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