- Captain America/Steve Rogers as Prince Corneilus
- Iron Man/Tony Stark as Gringo
- Black Widow/Natasha Romanoff as Thumblina
- Winter Soldier/Bucky Barnes as Berkeley Beetle
- Baron Helmut Zemo as Mr. Mole
- Agent 13/Sharon Carter as Thumblina's Mother
- Thunderbolt Ross as Grundel
- James Rhodes/War Machine as Jacquimo
- Vision as Mozo
- Scarlet Witch/Wanda Maximoff as Mrs. Rabbit
- Spider-Man/Peter Parker as Baby Bug
- Christine Everhart as Gnatty
- Ellen Brandt (from Iron Man 3) as Mrs. Toad
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