Bugs Bunny (from Looney Tunes) as Homestar Runner

Tweety Bird (from Looney Tunes) as Pom Pom

Daffy Duck (from Looney Tunes) as Strong Bad

Gary (from Spongebob Squarepants) as The Cheat

Odie (from Garfield) as Strong Mad

Goofy (from Mickey Mouse) as Strong Sad

Squidward (from Spongebob Squarepants) as Bubs

Lola Bunny (from Looney Tunes) as Marzipan

Version 2

Thomas the Tank Engine = Homestar Runner

Wallace (Wallace and Gromit) = Strong Bad

Elmo (Sesame Street) = Poopsmith

Grumpy (Snow White) = Strong Mad

Skarloey (Thomas) = Homsar

Emily (Thomas) = Marzipan

Big Bird (Sesame Street) = Homeschool Winner

Version 3

Top Cat as Homestar

Diesel 10 (Thomas) as Strong bad (Both red)