- Kitty Katswell (from T.U.F.F. Puppy) as Mrs. Brisby
- Bernard (from The Rescuers) as Jeremy
- The Chief (from T.U.F.F. Puppy) as Nicodemus
- Shining Armor (from My Little Pony: Friendship is Magic) as Mr. Ages
- Kala (from Tarzan) as Auntie Shrew
- Squidward Tentacles (from Spongebob Squarepants) as Justin
- Jack Rabbit (from T.U.F.F. Puppy) as Jenner
- Rabbit (from Skunk Fu!) as Sullivan
- Dudley Puppy (from T.U.F.F. Puppy) as Jonathan Brisby
- Sabor (from Tarzan) as Dragon the Cat
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