- Penelope Pitstop (from The Perils of Penelope Pitstop) as Susan/Ginormica
- Bullwinkle J. Moose (from The Adventures of Rocky and Bullwinkle) as B.O.B.
- Basil of Baker Street (from The Great Mouse Detective) as Dr. Cockroach Ph.D.
- Wally Gator as The Missing Link
- Willie the Giant (from Mickey Mouse) as Insectosaurus
- Lyle Van de Groot (from George of The Jungle) as Gallaxhar