- Catherine "Cat" Ballou as Cloe
- Kid Shelleen and Edward R. Furrow
- Clay Boone as Bo
- Jed as Orson
- The Sunrise Kid as Wade
- Professor Sam the Shade as Jon Arbuckle
- Jackson Two-Bears as Newton
- Frankie Ballou as The Police Dog
- Sir Harry Percival as Alyosius
- Sheriff Cardigan as Garfield
- The Strawn as The Weasel
- Butch Cassidy as Plato
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