- Catherine "Cat" Ballou as Princess Daisy
- Kid Shelleen and Luigi
- Clay Boone as Yoshi
- Jed as Mario
- The Sunrise Kid as Wario
- Professor Sam the Shade as Toadsworth
- Jackson Two-Bears as Mallow
- Frankie Ballou as Mighty Plumber
- Sir Harry Percival as Bowser Jr.
- Sheriff Cardigan as Donkey Kong
- The Strawn as Bowser
- Butch Cassidy as Toad
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