• Charlie Chan as Mario
  • Yvonne Roland as Princess Peach
  • Betty Adams as Princess Daisy
  • Richard Masters as Luigi
  • Lee Chan as Yoshi
  • Arthur Hughes as Diddy Kong
  • Mr. Cartwright as Sir Ebrum
  • Charlie Chan, Jr. as Wario
  • Mr. Hopkins as Toad
  • Honorable Charles Zaraka as Oogtar
  • Inspector Strasser as Donkey Kong
  • Dr. Burton as Mighty Plumber

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