- Charlie Chan as Mario
- Yvonne Roland as Princess Peach
- Betty Adams as Princess Daisy
- Richard Masters as Luigi
- Lee Chan as Yoshi
- Arthur Hughes as Diddy Kong
- Mr. Cartwright as Sir Ebrum
- Charlie Chan, Jr. as Wario
- Mr. Hopkins as Toad
- Honorable Charles Zaraka as Oogtar
- Inspector Strasser as Donkey Kong
- Dr. Burton as Mighty Plumber
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