- Clarence as Mario
- Sumo as Luigi
- Breehn as Prince Peasely
- Ms. Shoop as Queen Bean
- Lil' Grandpa as Toadsworth
- Amy Gillis as Peach
- Ms. Julep (From The Substitute) as Lady Lima
- Joshua as Bowser/Rookie
- Ms. Lofton as Cackleta
- Unnamed Hooded Kid (From Spooky Boo) as Fawful
- Dustin as Popple
- Sandy (From Hairence) as Bowletta
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