- Flint Lockwood as Mickey Mouse
- Tall Dexter (from Dexter's Laboratory) as Donald Duck
- Tim Lockwood as Goofy
- Steve the Monkey as Pluto
- Samantha "Sam" Sparks as Minnie Mouse
- Roz (from Monsters, Inc.) as Daisy Duck
- Helen (from Horton Hears a Who) as Clarabelle Cow
- The Giant of Nod (from Dragon Tales) as Pete
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