- Numbuh 1 as Kermit
- Numbuh 362 as Miss Piggy
- Numbuh 4 as Fozzie Bear
- The Kid as Gonzo
- Maurice as Rowlf
- Numbuh 2 as Scooter
- Numbuh 5 as Skeeter
- Joey Beatles as Animal
- Tommy Gilligan as Bean Bunny
- Numbuh 3 as Janice
- Trevor as Dr. Bunsen Honeydew
- Henrietta Von Marzipan as Nanny
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