- Doctor N Gin as Phineas
- Doctor Nefarious Tropy as Ferb
- Coco Bandicoot as Isabella
- Tawna Bandicoot as Candace
- Pinstripe Potoroo as Jeremy
- Crash Bandicoot as Perry The Platypus
- Aku Aku as Major Monogram
- Doctor Neo Cortex as Doctor Heinz Doofenshmirtz
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