- Lieutenant Commander Ron Hunter as Mario
- Captain Frank Ramsey as Luigi
- Lieutenant Roy Zimmer as Yoshi
- Command Master Chief Walter Cob as Donkey Kong
- Lieutenant Peter Ince as Oogtar
- Lieutenant Bobby Dougherty as Diddy Kong
- Lieutenant Darik Westerguard as Mighty Plumber
- Officer of the Deck Mahoney as Toadsworth
- Chief of the Watch Hunsicker as Mallow
- Tactical Supervising Officer Billy Linkletter as Waluigi
- Petty Officer Third Class Russell Vossler as Wario
- Lieutenant Paul Hellerman as Boo
- and more
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