- Mike Church/Roman Strauss as Mickey Mouse
- Amanda Sharp/"Grace"/Margaret Strauss as Daisy Duck
- Gray Baker as Donald Duck
- Franklyn Madson as Scrooge McDuck
- "Piccolo" Pete Dugan as Professor Ludwig Von Drake
- Dr. Cozy Carlisle as Pete
- Inga as Clarabelle Cow
- Doug as Goofy
- Sister Madeleine as Minnie Mouse
- Father Timothy as Chip
- Frankie as Dale
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