- Mike Church/Roman Strauss as Kermit the Frog
- Amanda Sharp/"Grace"/Margaret Strauss as Annie Sue Pig
- Gray Baker as Fozzie Bear
- Franklyn Madson as Robin the Frog
- "Piccolo" Pete Dugan as Bobo the Bear
- Dr. Cozy Carlisle as Uncle Deadly
- Inga as Miss Piggy
- Doug as Animal
- Sister Madeleine as Janice
- Father Timothy as Dr. Bunsen Honeydew
- Frankie as Zoot
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