- Albert Johnson as Mario
- Sgt. Edgar Millen as Yoshi
- Constable Alvin Adams as Luigi
- George Washington Lincoln "Sundog" Brown as Diddy Kong
- Hazel as Wario
- Captain Hank Tucker as Toadsworth
- Vanessa McBride as Princess Peach
- Bill Luce as Donkey Kong
- Ned Warren as Toad
- Constable Hawkins as K. Lumsy
- Lewis as Zeekeeper
- Clarence as Bowser
- Deak De Bleargue as Baby Mario
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