• Albert Johnson as Mario
  • Sgt. Edgar Millen as Yoshi
  • Constable Alvin Adams as Luigi
  • George Washington Lincoln "Sundog" Brown as Diddy Kong
  • Hazel as Wario
  • Captain Hank Tucker as Toadsworth
  • Vanessa McBride as Princess Peach
  • Bill Luce as Donkey Kong
  • Ned Warren as Toad
  • Constable Hawkins as K. Lumsy
  • Lewis as Zeekeeper
  • Clarence as Bowser
  • Deak De Bleargue as Baby Mario

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