• Albert Johnson as Kermit the Frog
  • Sgt. Edgar Millen as Rizzo the Rat
  • Constable Alvin Adams as Gonzo the Great
  • George Washington Lincoln "Sundog" Brown as Dr. Teeth
  • Hazel as Beauregard
  • Captain Hank Tucker as Jim Henson
  • Vanessa McBride as Miss Piggy
  • Bill Luce as Sam the Eagle
  • Ned Warren as Dr. Bunsen Honeydew
  • Constable Hawkins as Beaker
  • Clarence as Uncle Deadly

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