• Steve Carell ... Alex as Gru (voice)
  • Jason Segel ... Dr Facilier as Vector (voice)
  • Russell Brand ... Danny as Dr. Nefario (voice)
  • Julie Andrews ... Gloria as Gru's Mom (voice)
  • Will Arnett ... Scar as Mr. Perkins (voice)
  • Kristen Wiig ... Zira as Miss Hattie (voice)
  • Miranda Cosgrove ... Penny as Margo (voice)
  • Dana Gaier ... Elsermrelda as Edith (voice)
  • Elsie Fisher ... Lilo as Agnes (voice)

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